BioChem - DOS

Candida zeylanoides as a new yeast model for lipid metabolism studies: effect of nitrogen sources on fatty acid accumulation

Folia microbiologica

da Rosa, Priscila Dallé Mattanna, Paula; Carboni, Dió rgenes; Amorim, Lucio; Richards, Neila; Valente, Patricia

<P>Lipid homeostasis is well-known in oleaginous yeasts, but there are few non-oleaginous yeast models apart from Saccharomyces cerevisiae. We are proposing the non-oleaginous yeast Candida zeylanoides QU 33 as model. The aim of this study was to investigate the influence of the carbon/nitrogen ratio and the type of nitrogen source upon oil accumulation by this yeast grown on shake flask cultures. The maximum biomass was obtained in yeast extract (2.39??0.19 g/l), followed by peptone (2.24??0.05 g/l), while the highest content of microbial oil (0.35??0.01 g/l) and the maximum lipid yield (15.63%) were achieved with peptone. Oleic acid was the predominant cellular fatty acid in all culture media (>32.23%), followed by linoleic (>15.79%) and palmitic acids (>13.47%). The highest lipid yield using glucose and peptone was obtained at the C/N ratio of 200:1.</P>

2014

Springer-Verlag

0015-5632

1874-9356

59

6

pp.477-484

10.1007/s12223-014-0325-3

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70612141