Combination of enzymatic hydrolysis and ethanol organosolv pretreatments: Effect on lignin structures, delignification yields and cellulose-to-glucose conversion

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바이오화학분류

- 바이오플라스틱
  1. 기타
- 바이오정밀화학
  1. 기타
- 화장품용 기능성소재
  1. 기타
- 의료용 화학소재
  1. 식품첨가제

논문

Combination of enzymatic hydrolysis and ethanol organosolv pretreatments: Effect on lignin structures, delignification yields and cellulose-to-glucose conversion

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Obama, Patrick; Ricochon, Guillaume; Muniglia, Lionel; Brosse, Nicolas

초록

Graphical abstract<ce:figure id='f0025'></ce:figure>Highlights► A 2-steps pretreatment process of miscanthus was investigated. ► The enzymatic pre-hydrolysis disrupted the lignocellulosic matrix. ► Lignin–holocellulose complex weakening enhanced the organosolv removal of lignin. AbstractEnzymatic pre-hydrolysis using the industrial enzymatic cocktail Cellulyve® was assessed as a first step in a pretreatment process of Miscanthus biomass involving an aqueous-ethanol organosolv treatment. 13C and 31P Nuclear Magnetic Resonance and size exclusion chromatography were used to analyze the cellulose and lignin before and after treatment. It was demonstrated that despite a very low impact on the fibre structure (observed by Scanning Electron Microscopy) and composition (in terms of sugars and polyphenolics content), the enzymatic pre-treatment disrupted the lignocellulosic matrix to a considerable extend. This weakening permitted enhanced removal of lignin during organosolv pulping and increased hydrolysability of the residual cellulosic pulp for the production of monomeric glucose. Using this combined treatment, a delignification yield of 93% and an enzymatic cellulose-to-glucose conversion of 75% were obtained.

발행연도

2012

라이선스

cc-by-nc-nd

ISSN

0960-8524

권

112

페이지

pp.156-163

DOI

10.1016/j.biortech.2012.02.080