BioChem - DOS

Growth, ethanol production, and inulinase activity on various inulin substrates by mutant Kluyveromyces marxianus strains NRRL Y-50798 and NRRL Y-50799

Journal of industrial microbiology & biotechnology

Galindo-Leva, Luz Á ngela; Hughes, Stephen R.; Ló pez-Nú ñ ez, Juan Carlos; Jarodsky, Joshua M.; Erickson, Adam; Lindquist, Mitchell R.; Cox, Elby J.; Bischoff, Kenneth M.; Hoecker, Eric C.; Liu, Siqing; Qureshi, Nasib; Jones, Marjorie A.

<P>Economically important plants contain large amounts of inulin. Disposal of waste resulting from their processing presents environmental issues. Finding microorganisms capable of converting inulin waste to biofuel and valuable co-products at the processing site would have significant economic and environmental impact. We evaluated the ability of two mutant strains of <I>Kluyveromyces marxianus</I> (Km7 and Km8) to utilize inulin for ethanol production. In glucose medium, both strains consumed all glucose and produced 0.40&nbsp;g ethanol/g glucose at 24&nbsp;h. In inulin medium, Km7 exhibited maximum colony forming units (CFU)/mL and produced 0.35&nbsp;g ethanol/g inulin at 24&nbsp;h, while Km8 showed maximum CFU/mL and produced 0.02&nbsp;g ethanol/g inulin at 96&nbsp;h. At 24&nbsp;h in inulin&nbsp;+&nbsp;glucose medium, Km7 produced 0.40&nbsp;g ethanol/g (inulin&nbsp;+&nbsp;glucose) and Km8 produced 0.20&nbsp;g ethanol/g (inulin&nbsp;+&nbsp;glucose) with maximum CFU/mL for Km8 at 72&nbsp;h, 40&nbsp;% of that for Km7 at 36&nbsp;h. Extracellular inulinase activity at 6&nbsp;h for both Km7 and Km8 was 3.7 International Units (IU)/mL.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1007/s10295-016-1771-5) contains supplementary material, which is available to authorized users.</P>

2016

Springer Berlin Heidelberg

1367-5435

1476-5535

43

pp.927-939

10.1007/s10295-016-1771-5

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART98373630

Kluyveromyces marxianus; Inulin; Inulinase; Coffee waste fermentation; Biorefinery platform