초록
<P><B>Abstract</B></P><P>An extractive fermentation approach in an aqueous two-phase system (ATPS) with wheat bran as a main substrate was used in this study to intensify the production of β-glucosidase enzyme (EC 3.2.1.21) from<I>Aspergillus niger</I>NRRL 322. Possible parameters, such as phase concentration and incubation period, were evaluated based on enzyme production. The fungus expressed high enzyme production, i.e. 948 U/ml after 96 h at 180 RPM when PEG4000 (12% w/w) and potassium phosphate (14% w/w) were used. This strategy of extractive fermentation with ATPS shows an increase in β-glucosidase enzyme yield of 948 U/ml compared with submerged fermentation, which gives a yield of 790 U/ml. β-Glucosidase yield in salt phase also increased by 1.2-fold compared with the submerged fermentation under the same conditions.</P>