High-level production of thermotolerant β-xylosidase of Aspergillus sp. BCC125 in Pichia pastoris: Characterization and its application in ethanol production
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
High-level production of thermotolerant β-xylosidase of Aspergillus sp. BCC125 in Pichia pastoris: Characterization and its application in ethanol production
A gene coding for thermotolerant β-xylosidase from Aspergillus sp. BCC125 was characterized. The recombinant enzyme was expressed in methylotrophic yeast Pichia pastoris KM71 and especially high yield of secreted enzyme was obtained. β-xylosidase possessed high enzyme efficiency (K<SUB>cat</SUB>/K<SUB>m</SUB>=198.8mM<SUP>-1</SUP>s<SUP>-1</SUP>) toward pNP-β-D-xylopyranoside (pNPβX) with optimal temperature and pH for activity of 60<SUP>o</SUP>C and pH 4.0-5.0, respectively. The identified β-xylosidase showed clear synergism with previously identified xylanase for hydrolysis of xylan in vitro as well as simultaneous saccharification and fermentation process (SSF) in vivo with Pichia stipitis.