Bioconversion of D-xylose to D-xylonate with Kluyveromyces lactis
메타 데이터
바이오화학분류
바이오플라스틱
기타
바이오정밀화학
기타
화장품용 기능성소재
기타
의료용 화학소재
식품첨가제
논문
Bioconversion of D-xylose to D-xylonate with Kluyveromyces lactis
학술지
Metabolic engineering
저자명
Nygard, Y.; Toivari, M.H.; Penttila, M.; Ruohonen, L.; Wiebe, M.G.
초록
d-Xylonate was produced from d-xylose using Kluyveromyces lactis strains which expressed the gene for NADP<SUP>+</SUP>-dependent d-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19+/-2g d-xylonatel<SUP>-1</SUP> was produced when K. lactis expressing xyd1 was grown on 10.5gd-galactosel<SUP>-1</SUP> and 40g d-xylosel<SUP>-1</SUP>. Intracellular accumulation of d-xylonate (up to ~70mg [gbiomass]<SUP>-1</SUP>) was observed. d-Xylose was metabolised to d-xylonate, xylitol and biomass. Oxygen could be reduced to 6mmolO<SUB>2</SUB>l<SUP>-1</SUP>h<SUP>-1</SUP> without loss in titre or production rate, but metabolism of d-xylose and xylitol were more efficient when 12mmolO<SUB>2</SUB>l<SUP>-1</SUP>h<SUP>-1</SUP> were provided. d-Xylose uptake was not affected by deletion of either the d-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1ΔXYL1 did not produce extracellular xylitol and produced more d-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1ΔXYL2 produced high concentrations of xylitol and significantly less d-xylonate than the xyd1 strain with the endogenous XYL2.