초록
<P><B>Abstract</B></P> <P>The suitability of molasses, Napier grass (<I>Pennisetum purpureum</I>), empty fruit bunches (EFB), palm oil mill effluent (POME), and glycerol waste as a co-substrate with <I>Chlorella</I> sp. TISTR 8411 biomass for biohythane production was investigated. Mono-digestion of <I>Chlorella</I> biomass had hydrogen and methane yield of 23–35 and 164–177 mL gVS<SUP>−1</SUP>, respectively. Co-digestion of <I>Chlorella</I> biomass with 2–6% TS of organic wastes was optimized for biohythane production with hydrogen and methane yield of 17–75 and 214–577 mL gVS<SUP>−1</SUP>, respectively. The hydrogen and methane yield from co-digestion of <I>Chlorella</I> biomass with molasses, POME, and glycerol waste was increased by 8–100% and 80–264%, respectively. The biohythane production of co-digestion of <I>Chlorella</I> was 6–11 L L-mixed waste<SUP>−1</SUP> with an optimal C/N ratio range of 19–41 and H<SUB>2</SUB>/CH<SUB>4</SUB> ratio range of 0.06–0.3. Co-digestion of <I>Chlorella</I> biomass was significantly improved biohythane production in term of yield, production rate, and kinetics.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Co-digestion <I>Chlorella</I> sp. biomass with organic wastes improved biohythane production. </LI> <LI> Hydrogen and methane yield of co-digestion was 17–75 and 214–577 mL gVS<SUP>−1</SUP>. </LI> <LI> Co-digestion has C/N ratio of 19–41 and H<SUB>2</SUB>/CH<SUB>4</SUB> ratio of 0.06–0.3. </LI> <LI> Co-digestion improved yield, production, C/N ratio, and kinetics. </LI> </UL> </P>