BioChem - DOS

Improved production of 2,5-furandicarboxylic acid by overexpression of 5-hydroxymethylfurfural oxidase and 5-hydroxymethylfurfural/furfural oxidoreductase in Raoultella ornithinolytica BF60

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Yuan, Haibo; Li, Jianghua; Shin, Hyun-dong; Du, Guocheng; Chen, Jian; Shi, Zhongping; Liu, Long

<P><B>Abstract</B></P> <P>2,5-Furandicarboxylic acid (FDCA) is a promising bio-based building block and can be produced by biotransformation of 5-hydroxymethylfurfural (HMF). To improve the FDCA production, two genes&mdash;one encoding HMF oxidase (HMFO; from <I>Methylovorus</I> sp. strain MP688) and another encoding for HMF/Furfural oxidoreductase (HmfH; from <I>Cupriavidus basilensis</I> HMF14)&mdash;were introduced into <I>Raoultella ornithinolytica</I> BF60. The FDCA production in the engineered whole-cell biocatalyst increased from 51.0 to 93.6mM, and the molar conversion ratio of HMF to FDCA increased from 51.0 to 93.6%.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>R. ornithinolytica</I> BF60 can catalyze HMF into less toxic HMF alcohol. </LI> <LI> <I>R. ornithinolytica</I> BF60 can re-oxidize HMF alcohol to 2,5-furandicarboxylic acid (FDCA). </LI> <LI> Overexpressing HmfH and HMFO enzymes significantly improved FDCA titer and yield. </LI> </UL> </P>

2018

Elsevier

0960-8524

247

pp.1184-1188

10.1016/j.biortech.2017.08.166

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART79612530

2,5-Furandicarboxylic acid; Biotransformation; Raoultella ornithinolytica BF60; 5-Hydroxymethylfurfural oxidase; HMF/furfural oxidoreductase