초록
<P>Highly efficient hydrogen-producing anaerobic microflora was enriched from anaerobic sludge obtained from a methane fermentation plant, with soluble starch as substrate. The effect of culture medium pH on hydrogen production capacity and microbial community was investigated using a batch reactor equipped with a pH controller. The optimal pH was 5.0, the hydrogen yield was 2.59 mol/mol-glucose, and the maximum hydrogen production rate was 123 mL/L-culture/h. Partial 16S rDNA sequencing placed the isolated strain within the<I>Clostridiaceae</I>family. When the isolated strain adhered to polymer resin, the change in free energy of interaction was negative, indicating that the bacteria-resin interaction is thermodynamically favorable, and that the strain will readily immobilize on the resin.</P>