BioChem - DOS

Mutation breeding of lycopene-producing strain Blakeslea trispora by a novel atmospheric and room temperature plasma (ARTP)

Applied biochemistry and biotechnology

Qiang, Wang; Ling-ran, Feng; Luo, Wei; Han-guang, Li; Lin, Wang; Ya, Zhou; Xiao-bin, Yu

<P>To improve the fermentation efficiency of lycopene, a plasma jet, driven by an active helium atom supplied with atmospheric and room temperature plasma (ARTP) biological breeding system, was used as a new method to generate mutations in Blakeslea trispora (-). After several rounds of screening, a mutant A5 with high concentration of lycopene and dry biomass was isolated, which showed a maximum lycopene concentration (26.4 0.2 mg/g dry biomass) which was 55 % higher than the parent strain (16.9 0.3 mg/g dry biomass) in the production of lycopene. Compared with parent strain, B. trispora A5 required less dissolved oxygen (10 % less than that of parent strain) to reach maximum concentration in a 5-L stirred tank reactor batch fermentation.</P>

2014

Springer-Verlag

0273-2289

1559-0291

174

1

pp.452-460

10.1007/s12010-014-0998-8

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70406677