BioChem - DOS

Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture

Journal of biotechnology

Arifin, Yalun; Sabri, Suriana; Sugiarto, Haryadi; Krö mer, Jens O.; Vickers, Claudia E.; Nielsen, Lars K.

<P><B>Abstract</B></P><P>Sucrose has several advantages over glucose as a feedstock for bioprocesses, both environmentally and economically. However, most industrial <I>Escherichia coli</I> strains are unable to utilize sucrose. <I>E. coli</I> W can grow on sucrose but stops growing when sucrose concentrations become low. This is undesirable in fed-batch conditions where sugar levels are low between feeding pulses. Sucrose uptake rates were improved by removal of the <I>cscR</I> gene, which encodes a protein that represses expression of the sucrose utilization genes at low sucrose concentrations. Poly-3-hydroxybutyrate (PHB) was used as a model compound in order to assess the effect of improved sugar utilization on bio-production. In the <I>cscR</I> knockout strain, production from sucrose was improved by 50%; this strain also produced 30% more PHB than the wild-type using glucose. This result demonstrates the feasibility of utilizing sucrose as an industrial feedstock for <I>E. coli</I>-based bioprocesses in high cell density culture.</P>

2011

Elsevier

0168-1656

1873-4863

156

4

pp.275-278

10.1016/j.jbiotec.2011.07.003

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58045078

Sucrose; Escherachia coli W; Poly-3-hydroxybutyrate; Fermentation; Fed-batch; Industrial biotechnology