BioChem - DOS

Butanol production from hemicellulosic hydrolysate of corn fiber by a Clostridium beijerinckii mutant with high inhibitor-tolerance

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Guo, T.; He, A.y.; Du, T.f.; Zhu, D.w.; Liang, D.f.; Jiang, M.; Wei, P.; Ouyang, P.k.

A Clostridium beijerinckii mutant RT66 with considerable inhibitor-tolerance obtained by continuous culture was used for butanol production from non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid (SAHHC). In fed-batch fermentation, 1.8L of diluted SAHHC containing 10g/L of reducing sugar was provided during the acidogenic phase and 0.2L of concentrated SAHHC containing 300g/L of reducing sugar was provided during the solventogenic phase. The mutant produced a total amount of solvents of 12.9g/L, which consisted of 3.1g/L of acetone, 9.3g/L of butanol and 0.5g/L of ethanol. A solvent yield of 0.35g/g sugar and a productivity of 0.18g/Lh in 72h were achieved. The remarkable inhibitor-tolerance of C. beijerinckii RT66 demonstrates that this may be an excellent strain for butanol production from ligocellulosic materials.

2013

Elsevier Applied Science

0960-8524

135

pp.379-385

10.1016/j.biortech.2012.08.029

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65836477

Butanol; Clostridium beijerinckii; Lignocellulosic materials; Inhibitor-tolerance