초록
<P><B>Abstract</B></P> <P>The simultaneous saccharification and co-fermentation of <I>Saccharomyces cerevisiae</I> and <I>Candida tropicalis</I> for ethanol production using H<SUB>2</SUB>O<SUB>2</SUB>-pretreated corn stover (CS) were performed in 250-mL or 500-mL Erlenmeyer flasks at various <I>S. cerevisiae</I>-<I>C. tropicalis</I> ratios (4:1, 3:1, 2:1, 1:1, 1:2, 1:3 and 1:4), fermentation temperatures (32 °C, 35 °C and 38 °C), inoculation ratios (8%, 12% and 16%), initial pH values (4.5, 5 and 5.5) and fermentation times (96 h, 120 h and 144 h). The optimum <I>S. cerevisiae</I>-<I>C. tropicalis</I> ratio derived from mono-factor design test was 3:1. According to orthogonal design experiment results, the optimum fermentation temperature, inoculation ratio, initial pH and fermentation time with a dry solid substrate content of 25% were 32 °C 12%, 5.0 and 144 h, respectively, and the maximum ethanol concentration was 10.924 g/100 mL. The cell growth, ethanol production and total substrate consumption in the process of simultaneous saccharification and co-fermentation conformed to logistical model, Luedeking-Piret model and general substrate consumption law of mono-strain fermentation, respectively. Collectively, <I>S. cerevisiae</I> and <I>C. tropicalis</I> simultaneously produced ethanol from xylose and glucose without interference, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> To optimize the technology condition of SSCF of <I>S. cerevisiae</I> and <I>C. tropicalis</I>. </LI> <LI> Glucose and xylose simultaneously were converted into bioethanol in the SSCF process. </LI> <LI> The maximum ethanol concentration is 10.924 g/100 mL by SSCF under the optimum conditions. </LI> <LI> The kinetics model of SSCF process conformed to that of mono-strain fermentation. </LI> </UL> </P>