초록
<P><B>Abstract</B></P> <P>The characteristics and kinetics of biohydrogen production with Ni<SUP>2+</SUP> using hydrogen-producing bacteria (HPB) obtained from cow dung were investigated in batch tests. The results indicated that cumulative biohydrogen production and hydrogen yield were enhanced with an increase of 0.3–6 mg/L in the Ni<SUP>2+</SUP> concentration at 35 °C and an initial pH of 6.0. The maximum cumulative biohydrogen production was 442.52 mL, and the maximum hydrogen yield was 2.05 mol H<SUB>2</SUB>/mol sucrose at 0.6 mg/L Ni<SUP>2+</SUP>. Compared with the control, the cumulative biohydrogen production with 0.6 mg/L Ni<SUP>2+</SUP> was doubled. The major soluble metabolites produced in this study were ethanol, acetate, and butyrate. In addition, the final pH was lower than the initial pH after the production of biohydrogen, and the substrate degradation efficiency was slightly enhanced with an increase in the Ni<SUP>2+</SUP> concentration from 0.3 to 9 mg/L. Modified Gompertz and Han–Levenspiel models were used to describe the effects of Ni<SUP>2+</SUP> on biohydrogen production, and the model predicted data fit the experimental results well. The mathematical constants <I>n</I> and <I>m</I> of these models were 103.59 and 2.69, respectively, suggesting that the reaction was uninhibited. The predicted critical Ni<SUP>2+</SUP> concentration was 15.58 mg/L, which completely suppressed biohydrogen production. This study contributes to the improvement of organic waste utilisation by HPB.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Kinetic models were used to describe the effects of Ni<SUP>2+</SUP> on biohydrogen production. </LI> <LI> The reaction was an uncompetitive inhibition with the existence of Ni<SUP>2+</SUP>. </LI> <LI> The predicted critical Ni<SUP>2+</SUP> concentration suppressed the reaction was 15.58 mg/L. </LI> </UL> </P>