BioChem - DOS

Engineering wild-type robust Pediococcus acidilactici strain for high titer L- and D-lactic acid production from corn stover feedstock

Journal of biotechnology

Yi, X.; Zhang, P.; Sun, J.; Tu, Y.; Gao, Q.; Zhang, J.; Bao, J.

Pediococcus acidilactici TY112 producing l-lactic acid and P. acidilactici ZP26 producing d-lactic acid, were engineered from the wild-type P. acidilactici DQ2 by ldhD or ldh gene disruption, and the robustness of the wild-type strain to the inhibitors derived from lignocellulose pretreatment was maintained well. In simultaneous saccharification and fermentation (SSF), 77.66gL<SUP>-1</SUP> of l-lactic acid and 76.76gL<SUP>-1</SUP> of d-lactic acid were obtained at 25% (w/w) solids content of dry dilute acid pretreated and biodetoxified corn stover feedstock. l- and d-Lactic acid yield and productivity were highly dependent on the inhibitor removal extent due to the significant down-regulation on the expressions of ldh and ldhD encoding lactate dehydrogenase by inhibitor, especially syringaldehyde and vanillin at the low concentrations. This study provided a prototype of industrial process for high titer l- and d-lactic acid production from lignocellulose feedstock.

2016

Elsevier Science Publishers

0168-1656

1873-4863

217

pp.112-121

10.1016/j.jbiotec.2015.11.014

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74649359

Pediococcus acidilactici; l-Lactic acid; d-Lactic acid; Lignocellulose; Inhibitor tolerance; Biodetoxification