BioChem - DOS

Production and purification of an extracellular lipolytic enzyme using ionic liquid-based aqueous two-phase systems

Green chemistry : an international journal and green chemistry resource : GC

Ventura, Só nia Patrí cia Marques; de Barros, Rafael Lemos Freire; de Pinho Barbosa, José Murillo; Soares, Cleide Mara Faria; Lima, Á lvaro Silva; Coutinho, Joã o Araú jo Pereira

<P>The ability of ionic liquid-based aqueous two-phase systems (ATPS) to purify lipase produced by fermentation is here evaluated and compared against conventional PEG-based ATPS systems. Four ionic liquids, chosen after screening of a larger number of ionic liquids are evaluated, with the maximum purification and higher recovery being obtained for the systems based on [C<SUB>8</SUB>mim]Cl. It is shown that IL-based ATPS have a performance superior to PEG-based ATPS for the purification of this enzyme.</P><BR><BR><P>Graphic Abstract</P><P>The successful use of IL-based ATPS as separation techniques for the extraction and purification of enzymes from a fermentation process<BR><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2gc16428k'><BR></P>

2012

The Royal Society of Chemistry

1463-9262

1463-9270

14

3

pp.734-740

10.1039/c2gc16428k

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58906186