Anburajan, Parthiban; Pugazhendhi, Arivalagan; Park, Jong-Hun; Sivagurunathan, Periyasamy; Kumar, Gopalakrishnan; Kim, Sang-Hyoun
초록
<P><B>Abstract</B></P> <P>This study investigated the effect of 5-hydroxymethylfurfural (5-HMF) on high-rate continuous fermentative H<SUB>2</SUB> production in a lab-scale fixed bed reactor (FBR) inoculated with mixed culture granules and fed with 15g/L galactose at a hydraulic retention time of 6h and at 37°C. During the 83days of operation, 5-HMF up to 2.4g/L was spiked into the feedstock. The maximum hydrogen production performance of 26.6L/L-d and 2.9mol H<SUB>2</SUB>/mol galactose<SUB>added</SUB> were achieved at 5-HMF concentration of 0.6g/L. 5-HMF concentration exceeding 0.9g/L not only inhibited hydrogen production but also affected the biofilm structure and microbial community population. However, when 5-HMF was eliminated from the feedstock, the performance and microbial community population were rapidly recovered.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 5-HMF at or over 0.9g/L inhibited continuous H<SUB>2</SUB> production from galactose. </LI> <LI> 5-HMF shifted dominant groups from <I>Clostridium butyricum</I> to <I>Lactobacillus</I>. </LI> <LI> Inhibition to continuous culture was milder than that to batch culture. </LI> <LI> Inhibition was rapidly overcome when 5-HMF was eliminated from feedstock. </LI> </UL> </P>