BioChem - DOS

Enzymatic hydrolysis of pretreated sugar cane bagasse using Penicillium funiculosum and Trichoderma harzianum cellulases

Process biochemistry

Maeda, Roberto Nobuyuki; Serpa, Viviane Isabel; Rocha, Vanessa Alves Lima; Mesquita, Renata Aparecida Alves; Anna, Lidia Maria Melo Santa; de Castro, Aline Machado; Driemeier, Carlos Eduardo; Pereira Jr., Nei; Polikarpov Jr., Igor

<P><B>Abstract</B></P><P>In this study, we investigated the enzymatic hydrolysis of pretreated sugarcane bagasse using eight different enzymatic blends obtained from concentrated crude enzyme extracts produced by <I>Penicillium funiculosum</I> and <I>Trichoderma harzianum</I> as well as from the extracts in combination with a commercial enzymatic cocktail. The influence of different levels of biomass delignification, degree of crystallinity of lignicellulose, composition of enzymatic activities and BSA on enzymatic hydrolysis yields (HYs) was evaluated. Our X-ray diffraction studies showed that crystallinity of lignocellulose is not a key determinant of its recalcitrance toward enzymatic hydrolysis. In fact, under the experimental conditions of our study, an increase in crystallinity of lignocellulosic samples resulted in increased glucose release by enzymatic hydrolysis. Furthermore, under the same conditions, the addition of BSA had no significant effect on enzymatic hydrolysis. The most efficient enzyme blends were obtained by mixing a commercial enzymatic cocktail with <I>P. funiculosum</I> or <I>T. harzianum</I> cellulase preparations (HYs above 97%) followed by the concentrated extract of <I>P. funiculosum</I> alone (HY=88.5%). Increased hydrolytic efficiencies appeared to correlate with having an adequate level of both &beta;-glucosidase and xylanase activities in the blends.</P>

2011

Elsevier

publisher-specific-oa

1359-5113

46

5

pp.1196-1201

10.1016/j.procbio.2011.01.022

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART55760835

Residual biomass; Sugar cane bagasse; Lignocellulosic materials; Enzymatic hydrolysis