Rodrigues, É llen Francine; Ficanha, Aline Matuella Moreira; Dallago, Rogé rio Marcos; Treichel, Helen; Reinehr, Christian Oliveira; Machado, Tainara Paula; Nunes, Greice Borges; Colla, Luciane Maria
초록
<P><B>Abstract</B></P> <P>The aim of this study was the production of amylolytic enzymes by solid state or submerged fermentations (SSF or SF, respectively), followed by purification using chemical process or microfiltration and immobilization of purified enzymes in a polyurethane support. The free and immobilized enzymes obtained were used to evaluate enzymatic hydrolysis of the polysaccharides of <I>Spirulina</I>. Microfiltration of the crude extracts resulted in an increase in their specific activity and thermal stability at 40°C and 50°C for 24h, as compared to extracts obtained by SSF and SF. Immobilization of polyurethane purified enzyme produced yields of 332% and 205% for the enzymes obtained by SF and SSF, respectively. Free or immobilized enzymes favor the generation of fermentable sugar, being the application of the purified and immobilized enzymes in the hydrolysis of microalgal polysaccharides considered a promising alternative towards development of the bioethanol production process from microalgal biomass.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Production of enzymes using agro industrial residuals. </LI> <LI> Proposition of a combined strategy of purification. </LI> <LI> Purification with membrane technology and immobilization. </LI> <LI> The enzymes presented thermal stability at 50°C. </LI> <LI> Possible use of enzymes in simultaneous saccharification and fermentation. </LI> <LI> Demonstration of use of enzymes in the saccharification of carbohydrate-rich biomass. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>