Enhancement of docosahexaenoic acid production by low-energy ion implantation coupled with screening method based on Sudan black B staining in Schizochytrium sp.

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메타 데이터

바이오화학분류

- 바이오정밀화학
  1. 화학제품
  2. 기타
- 화장품용 기능성소재
  1. 계면활성제⁄증점제
- 의료용 화학소재
  1. 치료제
  2. 건강보조식품

논문

Enhancement of docosahexaenoic acid production by low-energy ion implantation coupled with screening method based on Sudan black B staining in Schizochytrium sp.

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Fu, J.; Chen, T.; Lu, H.; Lin, Y.; Xie, X.; Tian, H.; Zheng, C.; He, D.

초록

Schizochytrium sp. is a hopeful docosahexaenoic acid (DHA) producing candidate due to its rapid growth rate and high DHA proportion in total lipid content. In this study, low-energy ion implantation was applied to Schizochytrium sp. to induce high DHA-producing mutants. Screening these mutants by Sudan black B staining, a mutant strain S1 which showed a 61% improvement in DHA production than that of the parent strain was successfully selected. Subsequently, parameters of DHA production of mutant strain S1 were optimized in a 500-mL Erlenmeyer flask. Under the optimum fermentation conditions, the production of DHA and the percentage of DHA in total lipid of mutant strain S1 were 6.52g/L and 46.2%, respectively. This study provides an effective breeding strategy for improved DHA production of Schizochytrium sp. through combination of the novel mutagenesis technology, the effective screening method and fermentation optimization.

발행연도

2016

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

221

페이지

pp.405-411

DOI

10.1016/j.biortech.2016.09.058