BioChem - DOS

Cell degeneration and performance decline of immobilized Clostridium acetobutylicum on bagasse during hydrogen and butanol production by repeated cycle fermentation

International journal of hydrogen energy

Liu, Jingyun; Zhou, Wencan; Fan, Senqing; Qiu, Boya; Wang, Yuyang; Xiao, Zeyi; Tang, Xiaoyu; Wang, Wenguo; Jian, Shizhao; Qin, Yangmei

<P><B>Abstract</B></P> <P>The cell degeneration and the fermentation performance decline during repeated cycle fermentation with immobilized <I>Clostridium acetobutylicum</I> on bagasse for hydrogen and butanol production was studied. The cell degeneration has been characterized in abnormality of a long-chain morphology through 7 cycles of repeated fermentation. The fermentation performance decline has been indicated by decrease of glucose consumption rate from 0.82 g/L/h to 0.22 g/L/h, reduction of hydrogen production and productivity from 6 L/L to 2.5 L/L and 170 mL/L/h to 40 mL/L/h, as well as the decrease of butanol production and butanol productivity from 6.5 g/L to 1.0 g/L and 0.18 g/L/h to 0.02 g/L/h, respectively. The ratio of hydrogen production and butanol production was in the range of 6&ndash;10 and 17&ndash;20 during the earlier three and later four cycles, respectively. The carbon flow directed to ethanol was higher during the later period of fermentation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cells morphology with long chains of cells performed during long-term fermentation. </LI> <LI> Cells degenerated and fermentation performance weakened with repeated cycle increase. </LI> <LI> Ratio of hydrogen to butanol increased with fermentation proceeding. </LI> <LI> Allocation of carbon element in the metabolites changed during repeated cycle fermentation. </LI> </UL> </P>

2019

Elsevier

0360-3199

1879-3487

44

48

pp.26204-26212

10.1016/j.ijhydene.2019.08.102

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART97998340

Degeneration; Hydrogen; Butanol; Repeated cycle fermentation; Immobilized cell