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Production and optimization of cellulase enzyme using Aspergillus niger USM AI 1 and comparison with Trichoderma reesei via solid state fermentation system

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논문

Production and optimization of cellulase enzyme using Aspergillus niger USM AI 1 and comparison with Trichoderma reesei via solid state fermentation system

학술지

Biotechnology research international

저자명

Lee, C. K.; Darah, I.; Ibrahim, C. O.

초록

<P>Novel design solid state bioreactor, FERMSOSTAT, had been evaluated in cellulase production studies using local isolate <I>Aspergillus niger</I> USM AI 1 grown on sugarcane bagasse and palm kernel cake at 1 : 1 (w/w) ratio. Under optimised SSF conditions of 0.5 kg substrate; 70% (w/w) moisture content; 30°C; aeration at 4 L/h · g fermented substrate for 5 min and mixing at 0.5 rpm for 5 min, about 3.4 U/g of Filter paper activity (FPase) was obtained. At the same time, comparative studies of the enzymes production under the same SSF conditions indicated that FPase produced by <I>A. niger</I> USM AI 1 was about 35.3% higher compared to <I>Trichoderma reesei</I>. This shows that the performance of this newly designed SSF bioreactor is acceptable and potentially used as prototype for larger-scale bioreactor design.</P>

발행연도

2011

발행기관

SAGE-Hindawi Access to Research

ISSN

2090-3138

ISSN

2090-3146

2011

페이지

pp.658493

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1 2023-12-11

논문; 2011-12-31

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