BioChem - DOS

A new β-glucosidase producing yeast for lower-cost cellulosic ethanol production from xylose-extracted corncob residues by simultaneous saccharification and fermentation

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Lewis Liu, Z.; Weber, Scott A.; Cotta, Michael A.; Li, Shi-Zhong

<P><B>Highlights</B></P><P>&#x025BA; An ethanologenic yeast able to utilize cellobiose as sole carbon source. &#x025BA; Produces sufficient &beta;-glucosidase activity for cellulosic ethanol production by SSF. &#x025BA; Tolerant to biomass pre-treatment inhibitors such as furfural and HMF. &#x025BA; Facilitates economic consolidated SSF bio-processing.</P> <P><B>Abstract</B></P><P>This study reports a new yeast strain of <I>Clavispora</I> NRRL Y-50464 that is able to utilize cellobiose as sole source of carbon and produce sufficient native &beta;-glucosidase enzyme activity for cellulosic ethanol production using SSF. In addition, this yeast is tolerant to the major inhibitors derived from lignocellulosic biomass pre-treatment such as 2-furaldehyde (furfural) and 5-(hydroxymethyl)-2-furaldehyde (HMF), and converted furfural into furan methanol in less than 12h and HMF into furan-2,5-dimethanol within 24h in the presence of 15mM each of furfural and HMF. Using xylose-extracted corncob residue as cellulosic feedstock, an ethanol production of 23g/l was obtained using 25% solids loading at 37&deg;C by SSF without addition of exogenous &beta;-glucosidase. Development of this yeast aids renewable biofuels development efforts for economic consolidated SSF bio-processing.</P>

2012

0960-8524

104

pp.410-416

10.1016/j.biortech.2011.10.099

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58196818

&beta; -Glucosidase; Cellobiose; Cellulosic ethanol; Corncobs; Simultaneous saccharification and fermentation;