BioChem - DOS

Aqueous two-phase systems based on cholinium salts and tetrahydrofuran and their use for lipase purification

Separation and purification technology

Souza, R.L.; Lima, R.A.; Coutinho, J.A.P.; Soares, C.M.F.; Lima, A.S.

Aqueous two-phase systems (ATPS) formed with cholinium-based ionic liquid - ILs (or salts) are a novel, low cost, and high efficient technique for the recovery of biomolecules. This study examines the formation of ATPS based on cholinium-based salts (cholinium chloride, cholinium bitartrate and cholinium dihydrogencitrate) and tetrahydrofuran (THF) for the purification of lipase from Bacillus sp. ITP-001, produced by submerged fermentation. The optimum conditions for this purification were determined to be 40wt% of THF and 30wt% of cholinium bitartrate at 25<SUP>o</SUP>C. A purification factor of 130.1+/-11.7 fold, a lipase yield of 90.0+/-0.7% and a partition coefficient of enzyme for IL-rich phase (K<SUB>E</SUB>=0.11+/-0.01) and protein contaminants for THF-rich phase (K<SUB>P</SUB>=1.16+/-0.1) were achieved.

2015

Elsevier Science B.V

1383-5866

155

pp.118-126

10.1016/j.seppur.2015.05.021

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART73287400

Aqueous two-phase systems; Lipase; Cholinium-based ionic liquid; Purification