BioChem - DOS

Effects of substrate loading and co-substrates on thermophilic anaerobic conversion of microcrystalline cellulose and microbial communities revealed using high-throughput sequencing

International journal of hydrogen energy

Xia, Y.; Cai, L.; Zhang, T.; Fang, H.H.P.

Batch tests were conducted to investigate the effect of co-substrates, including glucose, xylose and starch, on thermophilic anaerobic conversion of microcrystalline cellulose using mixed culture enriched from anaerobic digestion sludge (ADS). Up to 30.9% of cellulose was utilized with xylose as co-substrate. When using glucose as co-substrate, cellulose conversion rate reached the maximum of 0.048 g/l/h at cellulose loading of 5.0 g/l. Illumina high-throughput sequencing of the 16S rRNA gene revealed that the thermophilic consortium exclusively consisted of Clostridium (more than 70% of all sequences). Growth of Thermoanaerobacterium over Clostridium would inhibit cellulose conversion capacity of the consortium. But the growth of Thermoanaerobacterium could be repressed by pH higher than pH 6.0. Co-substrates caused noticeable variation of bacterial community structure. Predominance of Thermoanaerobacterium over Clostridium was observed when monosugars (glucose and xylose) were used as co-substrate without pH control. Starch was ineffective as co-substrate because it competed with cellulose for Clostridium.

2012

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

37

18

pp.13652-13659

10.1016/j.ijhydene.2012.02.079

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART63863967

Co-substrate; Microcrystalline cellulose; Thermophilic; Anaerobic; Illumina; High-throughput