Effect of biotin and a similar compound on succinic acid fermentation by Actinobacillus succinogenes in a chemically defined medium

BioChem - DOS

Search

메타 데이터

바이오화학분류

- 바이오플라스틱
  1. 고무
  2. 플라스틱
- 바이오정밀화학
  1. 용매
  2. 화학제품
- 화장품용 기능성소재
  1. 계면활성제⁄증점제
- 의료용 화학소재
  1. 건강보조식품
  2. 식품첨가제

논문

Effect of biotin and a similar compound on succinic acid fermentation by Actinobacillus succinogenes in a chemically defined medium

학술지

Biochemical engineering journal

저자명

Xi, Y.l.; Chen, K.q.; Xu, R.; Zhang, J.h.; Bai, X.f.; Jiang, M.; Wei, P.; Chen, J.y.

초록

A minimal chemically defined medium (CDM) was created and used to study the effect of biotin on the metabolism of Actinobacillus succinogenes NJ 113. The medium contained four amino acids and vitamins as key growth factors. To gain insight into the effect of biotin, different concentrations of this compound were added, and glucose utilisation, cell growth, and the succinic acid concentration were investigated. Anaerobic batch fermentation was carried out at optimised conditions in a 3L fermentor with a succinic acid concentration of 45.2g/L and a yield of 90.4%. A metabolic pathway was proposed after further study of the mechanism underlying the metabolism of biotin. The same results were obtained when 5-aminolevulinate (5-ALA) was used instead of biotin. We suggest that 5-ALA is a great alternative to biotin for the preparation of succinic acid by biological fermentation.

발행연도

2012

발행기관

Elsevier

ISSN

1369-703x

권

페이지

pp.87-92

DOI

10.1016/j.bej.2012.08.016

상세정보링크