Recycling Carbon Dioxide during Xylose Fermentation by Engineered Saccharomyces cerevisiae
메타 데이터
바이오화학분류
바이오플라스틱
고무
플라스틱
기타
바이오정밀화학
용매
화학제품
연료
기타
화장품용 기능성소재
기능성
계면활성제⁄증점제
기타
의료용 화학소재
치료제
식품첨가제
논문
Recycling Carbon Dioxide during Xylose Fermentation by Engineered Saccharomyces cerevisiae
학술지
ACS Synthetic biology
저자명
Xia, Peng-Fei; Zhang, Guo-Chang; Walker, Berkley; Seo, Seung-Oh; Kwak, Suryang; Liu, Jing-Jing; Kim, Heejin; Ort, Donald R.; Wang, Shu-Guang; Jin, Yong-Su
초록
<P>Global climate change caused by the emission of anthropogenic greenhouse gases (GHGs) is a grand challenge to humanity. To alleviate the trend, the consumption of fossil fuels needs to be largely reduced and alternative energy technologies capable of controlling GHG emissions are anticipated. In this study, we introduced a synthetic reductive pentose phosphate pathway (rPPP) into a xylose-fermenting <I>Saccharomyces cerevisiae</I> strain SR8 to achieve simultaneous lignocellulosic bioethanol production and carbon dioxide recycling. Specifically, ribulose-1,5-bisphosphate carboxylase/oxygenase from <I>Rhodospirillum rubrum</I> and phosphoribulokinase from <I>Spinacia oleracea</I> were introduced into the SR8 strain. The resulting strain with the synthetic rPPP was able to exhibit a higher yield of ethanol and lower yields of byproducts (xylitol and glycerol) than a control strain. In addition, the reduced release of carbon dioxide by the engineered strain was observed during xylose fermentation, suggesting that the carbon dioxide generated by pyruvate decarboxylase was partially reassimilated through the synthetic rPPP. These results demonstrated that recycling of carbon dioxide from the ethanol fermentation pathway in yeast can be achieved during lignocellulosic bioethanol production through a synthetic carbon conservative metabolic pathway. This strategy has a great potential to alleviate GHG emissions during the production of second-generation ethanol.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/asbcd6/2017/asbcd6.2017.6.issue-2/acssynbio.6b00167/production/images/medium/sb-2016-001673_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/sb6b00167'>ACS Electronic Supporting Info</A></P>