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Enzymatic routes for the production of mono- and di-glucosylated derivatives of hydroxytyrosol

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논문

Enzymatic routes for the production of mono- and di-glucosylated derivatives of hydroxytyrosol

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Trincone, Antonio; Pagnotta, Eduardo; Tramice, Annabella

초록

<P><B>Highlights</B></P><P>&#x025BA; New eco-friendly syntheses of more hydrosoluble biophenols were proposed. &#x025BA; A marine &alpha;-glucosidase and a commercial tyrosinase from mushroom were used. &#x025BA; Hydroxytyrosol and tyrosol &alpha;-glycosidic derivatives were efficiently produced. &#x025BA; Antioxidant activity of hydroxytyrosol &alpha;-glycosylated derivatives was tested. &#x025BA; The most interesting product is the (3,4-dihydroxyphenyl)ethyl-&alpha;-<SMALL>D</SMALL>-glucopyranoside.</P> <P><B>Abstract</B></P><P>In this work, a new eco-friendly procedure for the synthesis of hydroxytyrosol and tyrosol &alpha;-glycosidic derivatives was proposed by using the marine &alpha;-glucosidase from <I>Aplysia fasciata</I>, and a commercial tyrosinase from mushroom for the bioconversion of tyrosol glycosidic derivatives into the corresponding hydroxytyrosol products. New hydroxytyrosol mono- and di-saccharide derivatives were synthesized at final concentrations of 9.35 and 10.8g/l of reaction, respectively, and their antioxidant activity was evaluated by DPPH test. The best antioxidant agent resulted the (3,4-dihydroxyphenyl) ethyl-&alpha;-<SMALL>D</SMALL>-glucopyranoside; it showed a radical scavenging activity similar to that of the hydroxytyrosol, together with an increased hydrosolubility. This molecule could be a good response to many food industry demands, always in search of cheap antioxidants with nutritional properties to improve the nutritional value and the quality of foods.</P>

발행연도

2012

ISSN

0960-8524

115

페이지

pp.79-83

주제어

&alpha; -Glucosidase; Aplysia fasciata; Transglycosylation; Hydroxytyrosol; Biocatalysis;

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논문; 2012-07-01

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