H2-driven biotransformation of n-octane to 1-octanol by a recombinant Pseudomonas putida strain co-synthesizing an O2-tolerant hydrogenase and a P450 monooxygenase

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논문

H2-driven biotransformation of n-octane to 1-octanol by a recombinant Pseudomonas putida strain co-synthesizing an O2-tolerant hydrogenase and a P450 monooxygenase

학술지

Chemical communications : Chem comm

저자명

Lonsdale, Thomas H.; Lauterbach, Lars; Honda Malca, Sumire; Nestl, Bettina M.; Hauer, Bernhard; Lenz, Oliver

초록

An in vivo biotransformation system is presented that affords the hydroxylation of n-octane to 1-octanol on the basis of NADH-dependent CYP153A monooxygenase and NAD+-reducing hydrogenase heterologously synthesized in a bacterial host. The hydrogenase sustains H2-driven NADH cofactor regeneration even in the presence of O2, the co-substrate of monooxygenase. Graphic AbstractA bacterial whole-cell system was designed for hydroxylation of n-octane to 1-octanol at the expense of molecular hydrogen and oxygen. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5cc06078h'>

발행연도

2015

발행기관

The Royal Society of Chemistry

ISSN

1359-7345

ISSN

1364-548x

권

호

페이지

pp.16173-16175