BioChem - DOS

A microbial transformation using Bacillus subtilis B7-S to produce natural vanillin from ferulic acid

Scientific reports

Chen, Peng; Yan, Lei; Wu, Zhengrong; Li, Suyue; Bai, Zhongtian; Yan, Xiaojuan; Wang, Ningbo; Liang, Ning; Li, Hongyu

<P><I>Bacillus subtilis</I> strain B7-S screened from18 strains is an aerobic, endospore-forming, model organism of Gram-positive bacteria which is capable to form vanillin during ferulic acid bioconversion. The bioconversion of ferulic acid to vanillin by <I>Bacillus subtilis</I> B7-S (<I>B. subtilis</I> B7-S) was investigated. Based on our results, the optimum bioconversion conditions for the production of vanillin by <I>B. subtilis</I> B7-S can be summarized as follows: temperature 35 °C; initial pH 9.0; inoculum volume 5%; ferulic acid concentration 0.6 g/L; volume of culture medium 20%; and shaking speed 200 r/min. Under these conditions, several repeated small-scale batch experiments showed that the maximum conversion efficiency was 63.30% after 3 h of bioconversion. The vanillin products were confirmed by spectral data achieved from UV&#x2013;vis, inductively coupled plasma atomic emission spectroscope (ICP-AES) and Fourier transform infrared spectrometer (FT-IR) spectra. Scanning electron microscopy (SEM) and transmission electron spectroscopy (TEM) results confirmed that the cell surface of <I>B. subtilis</I> plays a role in the induction of ferulic acid tolerance. These results demonstrate that <I>B. subtilis</I> B7-S has the potential for use in vanillin production through bioconversion of ferulic acid.</P>

2016

Nature Publishing Group

cc-by

2045-2322

6

pp.20400

10.1038/srep20400

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75128668

https://www.nature.com/articles/srep20400.pdf