BioChem - DOS

Biosynthesis of pyruvic acid from glycerol-containing substrates and its regulation in the yeast Yarrowia lipolytica

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Kamzolova, Svetlana V.; Morgunov, Igor G.

<P><B>Abstract</B></P> <P>The ability of different yeasts to synthesize pyruvic acid (PA) from glycerol-containing substrates has been studied. The selected strain <I>Yarrowia lipolytica</I> VKM Y-2378 synthesized PA with &alpha;-ketoglutaric acid (KGA) as a byproduct. The content of KGA greatly depended on cultivation conditions. The minimal formation of the byproduct was provided by the limitation of yeast growth by thiamine (0.6 &micro;g/g biomass); the use of ammonium sulfate (0.6%) as a nitrogen source; addition of glycerol to cultivation medium in 20 g/L portions; maintaining the cultivation temperature at 28 &deg;C, pH of the cultivation medium at 4.5, and medium aeration between 55 and 60% of saturation; the optimal cultivation time was 48 h. The selected strain cultivated under such conditions in a fermenter with a waste glycerol from biodiesel production process synthesized 41 g/L PA with a yield of 0.82 g/g. The mechanism of PA production from glycerol-containing substrates in <I>Y. lipolytica</I> is discussed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pyruvic acid and its salts are commercially important biotechnological products. </LI> <LI> Biosynthesis of pyruvic acid from glycerol-containing substrates. </LI> <LI> Optimization of cultivation medium and nutrition medium. </LI> <LI> Efficient process of pyruvic acid production by <I>Yarrowia lipolytica.</I> </LI> </UL> </P>

2018

Elsevier

0960-8524

266

pp.125-133

10.1016/j.biortech.2018.06.071

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART86695042

Pyruvic acid (PA) production; Yarrowia lipolytica; Waste glycerol from biodiesel production process; Optimization