초록
<P><B>Abstract</B></P> <P>In the present study the whole slurry obtained after different pretreatment methods applied on sunflower straw (SS) biomass, was used for biological production of hydrogen (BHP) and ethanol (BEP). BHP from pretreated SS was studied in batch experiments using mixed anaerobic sludge, via simultaneous saccharification and fermentation (SSF), while for BEP, SSF was carried out using the xylose – fermenting yeast <I>P<I>ichia</I> stipitis</I>. The experimental results obtained showed that for BHP, alkaline pretreatment with 20g NaOH/100 gTS was optimal, leading to the highest hydrogen yield of 155.3±6.5L/kg TS. This yield was 52.5 times higher than the respective of raw SS (2.9±0.0 LH<SUB>2</SUB>/kgTS) without enzyme addition. Pretreatment with the same chemical loadings of HCl or H<SUB>2</SUB>SO<SUB>4</SUB> resulted in the lowest BHP yields, due to the generation of inhibitory compounds. Although pretreatment with 20g H<SUB>2</SUB>SO<SUB>4</SUB>/100gTS also caused inhibition of <I>P. stipitis</I> giving the lowest BEP yield, pretreatment with 20g HCl/100 gTS led to the highest BEP yield of 104.78±0.08mg ethanol/gTS, indicating the varying sensitivity of different microbial species to the inhibitory compounds that are generated during pretreatment.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 20 g NaOH/100gTS led to the highest BHP yield of 155.3±6.5L/kg TS. </LI> <LI> 20g HCl-H<SUB>2</SUB>SO<SUB>4</SUB> /100gTS resulted in the lowest BHP yields, due to inhibition. </LI> <LI> 20g HCl/100gTS led to the highest BEP yield of 104.78±0.08mg ethanol/gTS. </LI> <LI> P. stipitis was sensitive in high acetic acid and 5-HMF concentration. </LI> <LI> Different sensitivity of microbial species to the inhibitory compounds. </LI> </UL> </P>