초록
<P>For obtaining hypercellulolytic strains, Trichoderma reesei Rut C30 was treated by UV irradiation. After twice selection and 7 subcultures conduction, A5, A9, A13 and A22 four mutant strains were abtained, and their filter paper activity (FPA) increased 31.01%, 29.83%, 55.35%, 32.27% respectively than parent strain. Random amplified polymorphic DNA analysis (RAPD) demonstrated there was existed genetic diversity between the mutants. The maximum filter paper activity (FPA) of A13 achieved 6.09 U/ml by central composite design (CCD). For applying into poplar hydrolysis, the mixed cellulase of A13 and Eupenicillium javanicum ZN-205 resulted in superior production of reducing sugar than its single cellulase counterparts. The maximum reducing sugar production was 25.75g/L in 5% substrate, and the corresponding saccharification rate was 78.18%.</P>