초록
<P><B>Abstract</B></P> <P>This study proposes a dynamic model that describes key characteristics of fermentative butanol production from glucose and xylose mixtures. The model has 12 parameters and incorporates noncompetitive inhibitory interaction between sugars as well as inhibitions due to high substrate and butanol concentrations. Different pre-growth strategies to achieve co-utilization of sugars were explored together with their effects on fermentation kinetics. Mixed sugar fermentation by the cultures pre-grown on a mixture of glucose and xylose showed a higher endurance to inhibition, a 2-fold increase in butanol production and a 1.5-fold increase in total sugar consumption compared to cultures pre-grown on xylose only. The average squared correlation coefficients (r<SUP>2</SUP>) between experimental observations and model predictions were 0.917 and 0.926 for fermentations done by the cultures pre-grown on xylose only, and pre-grown on a mixture of glucose and xylose, respectively. Sensitivity analysis on the model parameters revealed that the growth parameters were the most critical. The proposed model can serve as a basis for modeling of microbial butanol production from lignocellulosic biomass and be applied to other substrates and microorganisms.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The model describes fermentation of glucose and xylose mixtures to produce butanol. </LI> <LI> Inhibitions due to interaction of sugars, high substrate and butanol are considered. </LI> <LI> Model parameters are estimated using data from our experiments and from literature. </LI> <LI> Effects of different pre-growth strategies on kinetics are investigated. </LI> <LI> Critical parameters are identified with a sensitivity analysis. </LI> </UL> </P>