A metagenomics approach for new biocatalyst discovery: application to transaminases and the synthesis of allylic amines
메타 데이터
바이오화학분류
바이오플라스틱
기타
바이오정밀화학
기타
화장품용 기능성소재
기타
의료용 화학소재
식품첨가제
논문
A metagenomics approach for new biocatalyst discovery: application to transaminases and the synthesis of allylic amines
학술지
Green chemistry : an international journal and green chemistry resource : GC
저자명
BaudJoint first authors., Damien; Jeffries, Jack W. E.; Moody, Thomas S.; Ward, John M.; Hailes, Helen C.
초록
<P>Transaminase enzymes have significant potential for the sustainable synthesis of amines using mild aqueous reaction conditions. Here a metagenomics mining strategy has been used for new transaminase enzyme discovery. Starting from oral cavity microbiome samples, DNA sequencing and bioinformatics analyses were performed. Subsequent <I>in silico</I> mining of a library of contiguous reads built from the sequencing data identified 11 putative Class III transaminases which were cloned and overexpressed. Several screening protocols were used and three enzymes selected of interest due to activities towards substrates covering a wide structural diversity. Transamination of functionalized cinnamaldehydes was then investigated for the production of valuable amine building blocks.</P><P>Graphic Abstract</P><P>A metagenomics mining strategy was used to identify new transaminases that were utilised in the synthesis of allylic amines.<BR/><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c6gc02769e'/><BR/></P>