초록
<P><B>Abstract</B></P> <P>In this study, stepwise increases in linalool production were obtained by combining metabolic engineering and process optimization of an unconventional oleaginous yeast <I>Yarrowia lipolytica</I>. The linalool synthetic pathway was successfully constructed by heterologously expressing a codon-optimized linalool synthase gene from <I>Actinidia arguta</I> in <I>Y. lipolytica.</I> To enhance linalool productivity, key genes involved in the mevalonate pathway were overexpressed in different combinations. Moreover, the overexpression of mutant <I>ERG20</I> <SUP>F88W-N119W</SUP> gene resulted in further linalool production. A maximum linalool titre of 6.96±0.29mg/L was achieved in shake flasks, which was the highest level ever reported in yeasts.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Y. lipolytica</I> was metabolically engineered to produce linalool. </LI> <LI> Overexpression of genes in MVA pathway and <I>IDI1</I> enhanced linalool production. </LI> <LI> Overexpressing <I>ERG20</I> <SUP>F88W-N119W</SUP> gene further led to a high linalool yield. </LI> <LI> Citrate was beneficial to linalool accumulation in <I>Y. lipolytica</I>. </LI> </UL> </P>