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Production of alkali tolerant cellulase free in high levels by Bacillus pumilus SV-205

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논문

Production of alkali tolerant cellulase free in high levels by Bacillus pumilus SV-205

학술지

International journal of biological macromolecules

저자명

Nagar, Sushil; Mittal, Anuradha; Kumar, Davender; Gupta, Vijay Kumar

초록

<P><B>Highlights</B></P><P>&#x025BA; Hyper level of xylanase under optimized conditions. &#x025BA; Highly alkali stable over a broad pH range of 6&ndash;11 for 24h. &#x025BA; Retained 65% activity after an incubation of 2h at 60&deg;C. &#x025BA; Parametric optimization leads to 21.63 fold increase in activity.</P> <P><B>Abstract</B></P><P>The fermentation conditions were optimized for hyper production of xylanase from <I>Bacillus pumilus</I> SV-205. The bacterium secretes high levels (7382.7&plusmn;1200IU/mL) of cellulase-free xylanase using wheat bran led to 21.63 fold increase in activity. A combination of yeast extract and peptone stimulated highest xylanase production (2448.0IU/mL) as compared to other combinations. The most important characteristic of the enzyme is its high pH stability (100%) over a broad pH range of 6&ndash;11 for 24h. Thermostability studies revealed that enzyme retained 65% activity after an incubation of 2h at 60&deg;C. The level of production is remarkable as compared to earlier reports.</P>

발행연도

2012

ISSN

0141-8130

ISSN

1879-0003

50

2

페이지

pp.414-420

주제어

Xylanase; Submerged fermentation; Bacillus pumilus; Wheat bran; Alkali stable

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1 2023-12-11

논문; 2012-12-31

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