초록
The purpose of this study is to elucidate the isomeric conversion of glucose to fructose using defatted microalga Dunaliella tertiolecta and Botryococcus braunii. Optimal temperature for immobilized-GI (Imm-GI) activity was determined to be 60<SUP>o</SUP>C. Under optimal conditions, glucose liberated from the defatted microalgal biomasses was ketoisomerically converted to fructose in time dependent manner, identified by high performance anion-exchange chromatography equipped with a pulsed electrochemical detector (HPAEC-PED). The specific activity of Imm-GI toward sugars of microalgal biomasses was determined to be 0.69mmol/mg for B. braunii and 0.83mmol/mg for D. tertiolecta, respectively. The calculated value for Imm-GI for the apparent equilibrium constant, K<SUP>'</SUP><SUB>eq</SUB>=0.96+/-0.03, at pH 6.0 and at optimum temperature 60<SUP>o</SUP>C. Collectively, our proposed approach first demonstrates the contributions and potential applications of Imm-GI for the isomeric conversion of glucose liberated from defatted microalgal biomasses as a major monosaccharide to fructose.