초록
The ability of Clostridium beijerinckii CG1 to utilize sugar from cane sugar factory wastewater (CSFW) and cellulosic ethanol pilot plant wastewater (CEPW) as a renewable carbon source and complete fermentation medium to produce acetone-butanol-ethanol (ABE) was investigated. CSFW and CEPW were used as the unsupplemented culture media (50mL) for ABE production by C. beijerenckii CG1 fermentation in 100-mL serum bottles. No acetone was produced from fermentation of either CEPW or CSFW, in contrast to the small amount (20g L<SUP>-1</SUP>) produced from glucose in the last 24h of a 120h fermentation. The average ethanol, butanol and butyric acid production from CEPW (butanol 0.6g L<SUP>-1</SUP>, ethanol 0.4g L<SUP>-1</SUP> and butyric acid 2.5g L<SUP>-1</SUP>) was higher than that from CSFW (butanol 0.3g L<SUP>-1</SUP>, ethanol 0.3g L<SUP>-1</SUP> and butyric acid 0.9g L<SUP>-1</SUP>), but still less than that from glucose (butanol 1.87g L<SUP>-1</SUP>, ethanol 0.86g L<SUP>-1</SUP> and butyric acid 6.47g L<SUP>-1</SUP>). However, the productivity rate in this 50-mL fermentation was three-fold higher with CSFW (0.06g L<SUP>-1</SUP> h<SUP>-1</SUP>) than with CEPW (0.02g L<SUP>-1</SUP> h<SUP>-1</SUP>). These results suggest that CSFW and CEPW can be applied as appropriate substrates to produce solvents by C. beijerinckii CG1 at a reasonable concentration without the need for additional exogenous nutrient supplements or pretreatment. The idea then being to consolidate the bioprocess for low-cost biofuel production.