BioChem - DOS

Enhancement of astaxanthin production from Haematococcus pluvialis mutants by three-stage mutagenesis breeding

Journal of biotechnology

Wang, N.; Guan, B.; Kong, Q.; Sun, H.; Geng, Z.; Duan, L.

Haematococcus pluvialis was modified for higher astaxanthin production compatible with the superiorities of high biomass and high activity by three-stage mutagenesis breeding. UV irradiation mutants named UV11-4 made an increase on cell dry weight, but showed a longer growth circle than the wild type. On the basis of UV mutants, ethyl methane sulphonate (EMS) mutants E2-5 cut down the latent phase, brought forward and extended the logarithmic phase. The inhibitor diphenylamine (DPA) was employed to screen high-yield astaxanthin producer by the color change of colonies from green to red on solid medium. Via the contravariant cultivation, proliferation and transformation, the mutant DPA12-2 possessed an 1.7-fold astaxanthin production compared to the wild type, reaching 47.21+/-3.30mg/g dry cells.

2016

Elsevier Science Publishers

0168-1656

1873-4863

236

pp.71-77

10.1016/j.jbiotec.2016.08.009

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART76321034

Haematococcus pluvialis; Astaxanthin; Three-stage mutagenesis; Diphenylamine