Enzymatic synthesis of L-tryptophan from hair acid hydrolysis industries wastewater with tryptophan synthase

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논문

Enzymatic synthesis of L-tryptophan from hair acid hydrolysis industries wastewater with tryptophan synthase

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Zhao, G.; Liu, J.; Dong, K.; Zhang, F.; Zhang, H.; Liu, Q.; Jiao, Q.

초록

An effective method for production of l-tryptophan from hair acid hydrolysis wastewater (HHW) containing l-serine was developed by recombinant tryptophan synthase. This study provides us with an alternative HHW utilization strategy. Tryptophan synthase could efficiently convert l-serine contained in HHW to l-tryptophan at pH 8.0, 40<SUP>o</SUP>C and Tween-80 of 0.04%. The enzyme also showed high tolerance to ammonium chloride, a component in HHW. In a scale up study, l-serine conversion rate reach 95.1% with a final l-tryptophan concentration of 33.2gl<SUP>-1</SUP>.

발행연도

2011

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

102

호

페이지

pp.3554-3557

DOI