BioChem - DOS

An ammonium-formate-driven trienzymatic cascade for ω-transaminase-catalyzed (R)-selective amination

Journal of organic chemistry

Chen, Fei-Fei; Zhang, Yu-Hui; Zhang, Zhi-Jun; Liu, Lei; Wu, Jian-Ping; Xu, Jian-He; Zheng, Gao-Wei

<P>(<I>R</I>)-Amination mediated by (<I>R</I>)-specific &omega;-transaminases generally requires costly <SMALL>D</SMALL>-alanine in excess to obtain the desired chiral amines in high yield. Herein, a one-pot, trienzymatic cascade comprising an (<I>R</I>)-specific &omega;-transaminase, an amine dehydrogenase, and a formate dehydrogenase was developed for the economical and eco-friendly synthesis of (<I>R</I>)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enabled efficient &omega;-transaminase-mediated (<I>R</I>)-amination of various ketones, with high conversions and excellent <I>ee</I> (>99%); water and CO<SUB>2</SUB> were the only waste products.</P><BR>[FIG OMISSION]</BR>

2019

American Chemical Society

0022-3263

1520-6904

84

22

pp.14987-14993

10.1021/acs.joc.9b02445

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