초록
<P><B>Abstract</B></P> <P>The <I>Klebsiella oxytoca</I> was engineered to produce 2,3-butanediol (2,3-BDO) simultaneously utilizing glucose and galactose obtained from a <I>Golenkinia</I> sp. hydrolysate. For efficient uptake of galactose at a high concentration of glucose, <I>Escherichia coli</I> galactose permease (GalP) was introduced, and the expression of <I>galP</I> under a weak-strength promoter resulted in simultaneous consumption of galactose and glucose. Next, to improve the sugar consumption, a gene encoding methylglyoxal synthase (MgsA) known as an inhibitor of multisugar metabolism was deleted, and the <I>mgsA</I>-null mutant showed much faster consumption of both sugars than the wild-type strain did. Finally, we demonstrated that the engineered <I>K. oxytoca</I> could utilize sugar extracts from a <I>Golenkinia</I> sp. hydrolysate and successfully produces 2,3-BDO.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Production of 2,3-BDO by <I>Klebsiella oxytoca</I> using <I>Golenkinia</I> sp. hydrolysates. </LI> <LI> Engineering of <I>Klebsiella oxytoca</I> for simultaneous utilization of mixed sugars. </LI> <LI> Optimization of gene expression using synthetic promoters. </LI> </UL> </P>