BioChem - DOS

Optimization of the Biotechnological Process for Hide Unhairing in Substitution of Toxic Sulfides

Chemical engineering & technology

Dettmer, A.; Coelho Cavalheiro, J.; Cavalli, É .; Misturini Rossi, D.; de Souza Gusatti, C.; Zá chia Ayub, M. A.; Gutterres, M.

<P><B>Abstract</B></P><P>Screening and isolation of a new <I>Bacillus subtilis</I> strain and production of its proteases for leather unhairing are described. <I>B. subtilis</I> strain BLBc 11 was isolated from the aerobic sludge of a tannery. Optimization of enzyme production by this bacterium was carried out using the Plackett&#8208;Burman and central composite design. Unhairing and inter&#8208;fibrillary removal capabilities were evaluated by scanning electron microscopy and determination of proteoglycans and glycosaminoglycans. Crude enzymatic extracts of <I>B. subtilis</I> BLBc 11 cultures were applied for the unhairing process of hides with excellent results, suggesting that this safe enzymatic preparation can replace the toxic chemicals commonly used in this process.</P>

2012

WILEY&#x2010;VCH Verlag

0930-7516

1521-4125

35

5

pp.803-810

10.1002/ceat.201100350

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART63135427

Bacillus subtilis; Hide unhairing; Leather production; Sulfide replacement