초록
<P><B>Abstract</B></P> <P>The aim of this work was to study cocktail supplementation for sugar cane bagasse hydrolysis, where the enzymes were provided from both commercial source and microorganism cultivation (<I>Trichoderma reesei</I> and genetically modified <I>Escherichia coli</I>), followed by purification. Experimental simplex lattice mixture design was performed to optimize the enzymatic proportion. The response was evaluated through hydrolysis microassays validated here. The optimized enzyme mixture, comprised of <I>T. reesei</I> fraction (80%), endoglucanase (10%) and β-glucosidase (10%), converted, theoretically, 72% of cellulose present in hydrothermally pretreated bagasse, whereas commercial Celluclast 1.5L converts 49.11%±0.49. Thus, a rational enzyme mixture designed by using synergism concept and statistical analysis was capable of improving biomass saccharification.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Well-balanced enzymatic cocktails as promising approach to optimize biomass hydrolysis. </LI> <LI> Sugar cane bagasse 5% (W/V) hydrolysis microassay was validated. </LI> <LI> Identification of necessary activities to improve fungal extract performance. </LI> <LI> Mixture experimental design was used to develop an optimized enzyme cocktail. </LI> <LI> Cellulose conversion was increased by approximately 40% in respect of Celluclast 1.5L. </LI> </UL> </P>