BioChem - DOS

Improving arachidonic acid accumulation in Mortierella alpina through B-group vitamin addition

Bioprocess and biosystems engineering

Zeng, Yan; Ji, Xiao-Jun; Chang, Shu-Mei; Nie, Zhi-Kui; Huang, He

<P>To improve the arachidonic acid (ARA) accumulation in Mortierella alpina, a mixed B-group vitamin addition strategy was developed. The ARA titer reached up to 10.0 g/L, 1.7-fold of the control. At the same time, the highest specific activities of key enzymes involved in ARA biosynthesis, including malic enzyme, glucose-6-phosphate dehydrogenase and ATP: citrate lyase, were 63.3, 38.6 and 53.7% higher than the control, respectively. The possible vitamin triggered improved ARA accumulation mechanism was thus elucidated that B-group vitamins could function as the cofactors of the key enzymes involved in ARA biosynthesis, or precursors for the formation of NADPH and acetyl-CoA which were crucial for ARA synthesis, and strengthened the related metabolic flux.</P>

2012

Springer-Verlag

1615-7591

1615-7605

35

5

pp.683-688

10.1007/s00449-011-0648-2

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART62423559