BioChem - DOS

Combined Detoxification and In-situ Product Removal by a Single Resin During Lignocellulosic Butanol Production

Scientific reports

Gao, Kai; Rehmann, Lars

<P><I>Phragmites australis</I> (an invasive plant in North America) was used as feedstock for ABE (acetone-butanol-ethanol) fermentation by <I>Clostridium saccharobutylicum</I>. Sulphuric acid pretreated phragmites hydrolysate (SAEH) without detoxification inhibited butanol production (0.73 g/L butanol from 30 g/L sugars). The treatment of SAEH with resin L-493 prior the fermentation resulted in no inhibitory effects and an ABE titer of 14.44 g/L, including 5.49 g/L butanol was obtained, corresponding to an ABE yield and productivity of 0.49 g/g and 0.60 g/L/h, respectively. Dual functionality of the resin was realized by also using it as an <I>in-situ</I> product removal agent. Integrating <I>in-situ</I> product removal allowed for the use of high substrate concentrations without the typical product inhibition. Resin-detoxified SAEH was supplemented with neat glucose and an effective ABE titer of 33 g/L (including 13.7 g/L acetone, 16.4 g/L butanol and 1.9 g/L ethanol) was achieved with resin-based <I>in-situ</I> product removal, corresponding to an ABE yield and productivity of 0.41 g/g and 0.69 g/L/h, respectively. Both detoxification of the substrate and the products was achieved by the same resin, which was added prior the fermentation. Integrating hydrolysate detoxification and <I>in-situ</I> butanol removal in a batch process through single resin can potentially simplify cellulosic butanol production.</P>

2016

Nature Publishing Group

cc-by

2045-2322

6

pp.30533

10.1038/srep30533

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART76159466

https://www.nature.com/articles/srep30533.pdf