BioChem - DOS

Bioprocess design guided by in situ substrate supply and product removal: Process intensification for synthesis of (S)-1-(2-chlorophenyl)ethanol

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Schmö lzer, Katharina; Mä dje, Katharina; Nidetzky, Bernd; Kratzer, Regina

<P>We report herein on bioprocess development guided by the hydrophobicities of substrate and product. Bioreductions of <I>o</I>-chloroacetophenone are severely limited by instability of the catalyst in the presence of aromatic substrate and (<I>S</I>)-1-(2-chlorophenyl)ethanol. <I>In situ</I> substrate supply and product removal was used to protect the utilized <I>Escherichia coli</I> whole cell catalyst based on <I>Candida tenuis</I> xylose reductase during the reaction. Further engineering at the levels of the catalyst and the reaction media was matched to low substrate concentrations in the aqueous phase. Productivities obtained in aqueous batch reductions were 21-fold improved by addition of 20% (v/v) hexane, NAD<SUP>+</SUP>, expression engineering, cell permeabilization and pH optimization. Reduction of 300&nbsp;mM substrate was accomplished in 97% yield and use of the co-solvent hexane in subsequent extraction steps led to 88% recovery. Product loss due to high catalyst loading was minimized by using the same extractant in bioreduction and product isolation.</P>

2012

Elsevier Applied Science, Elsevier Science Pub. Co

implied-oa

0960-8524

108

pp.216-223

10.1016/j.biortech.2012.01.009

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58595409

o-Chloroacetophenone bioreduction; Biocatalysis design cycle; Process intensification; In situ substrate supply; Product isolation