BioChem - DOS

Long-term production of butyric acid through immobilization of Clostridium tyrobutyricum in a moving fibrous-bed bioreactor (MFBB)

Journal of chemical technology and biotechnology

Shi, Zhouming; Huang, Lei; Wu, Xiangting; Luo, Lanping; Xiao, Kaijun; Cai, Jin; Xu, Zhinan

<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P>Butyric acid is an important chemical with wide industrial applications, but large&#8208;scale bio&#8208;production still requires an efficient bioprocess. For this purpose, one novel moving fibrous&#8208;bed bioreactor (MFBB) was developed in this work for economical production of butyric acid.</P><P><B>RESULTS</B></P><P>The cells of <I>Clostridium tyrobutyricum</I> were immobilized on a fibrous matrix packed in porous levitated sphere carriers. Each carrier was packed with a fiber quantity 2 cm &times; 6 cm (0.42 g) and was rotated at 100 rpm in a stirred&#8208;tank fermentor having pH and temperature controls. Batch and repeated&#8208;batch fermentations were carried out to produce butyric acid with a high yield (0.48 &plusmn; 0.015 g g<SUP>&#8208;1</SUP>). Subsequent fed&#8208;batch fermentation using the MFBB resulted in a high concentration of butyric acid (65.4 &plusmn; 1.4 g L<SUP>&#8208;1</SUP>). This novel MFBB could be employed to produce high concentration butyric acid (64.5 &plusmn; 1.2 g L<SUP>&#8208;1</SUP>) by a simple repeated&#8208;batch addition of a high concentration of glucose (>100 g L<SUP>&#8208;1</SUP>).</P><P><B>CONCLUSIONS</B></P><P>The MFBB system developed could practically and economically produce butyric acid from glucose for long&#8208;term operation. &copy; 2013 Society of Chemical Industry</P>

2014

John Wiley Sons, Ltd

0268-2575

1097-4660

89

12

pp.1883-1889

10.1002/jctb.4271

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70557850

butyric acid; moving fibrous&#x2010; bed bioreactor; Clostridium tyrobutyricum; immobilization;