초록
<P>To improve the utilization of sucrose for 2,3-butanediol (2,3-BD) production, four combinations of heterologous energy-conserving sucrose utilization pathways were introduced into <I>Bacillus subtilis</I> Δ<I>tet</I> strain (a derivative from <I>B. subtilis</I> 168) and <I>B. subtilis</I> FJ-1 strain (a new isolate in our lab). Results demonstrated that the combination of <I>cscB</I> (encoding sucrose permease) from <I>Escherichia coli</I> and <I>gtfA</I> (encoding sucrose phosphorylase) from <I>Streptococcus mutans</I> showed the most remarkable enhancement for the 2,3-BD production. With sucrose and sugar cane juice as substrate, respectively, the Δ<I>tet</I>-CEG strain (<I>B. subtilis</I> Δ<I>tet</I> strain containing the energy-conserving sucrose utilization pathway as referred above) showed 36.5% and 24.7% increase in 2,3-BD production than the control Δ<I>tet</I> strain, and the FJ-1-CEG strain also produced 23.8% and 44.5% more 2,3-BD than the control FJ-1 strain. The metabolic engineering strategy demonstrated in this study can be extensively applied to other microorganisms for reinforced production of desirable biochemicals from sucrose. Meanwhile, the newly isolated FJ-1 strain is an interesting platform strain that can be further engineered for efficient 2,3-BD production.</P><P>Overexpression of energy-conserving sucrose utilization pathways enables <I>Bacillus subtilis</I> for enhanced renewable 2,3-butanediol production from sucrose.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ascecg/2017/ascecg.2017.5.issue-12/acssuschemeng.7b03636/production/images/medium/sc-2017-03636k_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/sc7b03636'>ACS Electronic Supporting Info</A></P>